Ketosis is an important production disease and continues to cause significant economic losses to the farmers. Subclinical ketosis (SCK) causes greater losses than clinical ketosis because it occurs more frequently and often cannot be detected by farmers. In the present study a total of 108 early lactating buffaloes maintained in various organized and unorganized dairy farms in and around Bidar were screened to know the prevalence of subclinical ketosis. The findings of the present study revealed the overall prevalence of Subclinical ketosis in lactating buffaloes based on Precision xtra® blood BHBA meter was 14.81 per cent. Highest prevalence of SCK was recorded in third parity and least in first parity based on blood BHBA test. The biochemical study indicated statistically significant decrease in serum glucose, albumin, calcium and serum phosphorus, further statistically significant increase in AST in Subclinical ketosis affected buffaloes.