Vol. 10, Special Issue 7 (2025)

Author(s): Raghavendra Prasad Mishra, Barkha Sharma, Udit Jain, Parul, Sapna Tomar, Kapil Kumar Gupta and Devender Choudhary

Abstract: Mastitis is defined as an inflammatory reaction of the mammary gland induced when pathogenic microorganisms in the udder produce toxins that are harmful to the mammary gland. Mastitis is primarily caused by bacterial infection and is a major cause of economic loss in dairy cattle production. In the present study a total of 180 quarter milk samples were obtained from 85 cows at different areas of Mathura districts. All the milk samples were tested for subclinical mastitis by California Mastitis Test (CMT), isolation of E. coli was confirmed by using selective media (EMB) and biochemical tests (IMViC). Overall prevalence of SCM in the studied area was 18.82% (16/85). Prevalence of E. coli in all positive isolates, were 37.5% (6/16). For the prevention and control of mastitis, regular screening of SCM and milking hygiene practices together with awareness of farmers should be implemented.