Vol. 10, Special Issue 10 (2025)

Author(s): N Kowsalya and M Anandhi

Abstract: This experiment investigated the impact of applying short periods of incubation during egg storage (SPIDES), with or without turning, on the immune responses of broiler hatching eggs subjected to extended storage. A total of 750 eggs obtained from 33.5-week-old broiler breeders were allocated into five treatment groups: T₁ (control, no SPIDES), T₂ (three SPIDES cycles without turning), T₃ (three SPIDES cycles with three turns per cycle), T₄ (four SPIDES cycles without turning), and T₅ (four SPIDES cycles with three turns per cycle). Eggs were maintained at 17 °C and 75% relative humidity, while SPIDES-treated groups were intermittently exposed to 37.7 °C for three hours at five-day intervals. All eggs were stored for 21 days and then incubated on day 22. Results demonstrated that SPIDES application significantly (p?0.01) enhanced spleen weight; however, no notable effect was observed on Newcastle disease virus antibody titers at five weeks of age.

DOI: 10.22271/veterinary.2025.v10.i10Sa.2622