Vol. 9, Special Issue 2 (2024)

Author(s): AA Pathan, LC Modi, NF Chaudhari, AS Kardani, Falguni Modi and DV Bhut

Abstract: The purpose of this study was to see how the addition of Purslane (Portulaca oleracea) leaves extract to the Tris egg yolk citrate extender affected the sperm viability of cryopreserved Surti buck semen. A total of 64 semen samples were taken from four Surti bucks, with 16 samples collected twice a week via the artificial vagina method. To attain a final concentration of 100×10⁶ sperm/ml, the pooled sperm was diluted with the tris-egg yolk citrate extender and the Purslane (Portulaca oleracea) leaves aqueous extract was at varied concentrations: 0% (T₁), 1% (T₂), 2% (T₃), and 3% (T₄). The initial mean live sperm (%) and host reacted sperm (%) was non-significantly differed between all the groups. Pre-freeze and post thaw mean live sperm (%) and host reacted sperm (%) was significantly higher (p<0.01) in T₄ group as compared to T₁, T₂ and T₃ groups. Highest mean live sperm (%) and host reacted sperm (%) was found at initial, pre-freeze and post-thaw stage in T₄ group followed by T₃, T₂ and T₁ group.