Vol. 9, Special Issue 2 (2024)

Author(s): Yashkumar Hasmukhbhai Vaghasiya, Chandrakant F Chaudhari, Ramesh D Joshi, Tarun V Sutaria, Ravaji K Chaudhari, Bharat G Chaudhary, Manoj Sureshbhai Trivedi and Ravi D Prajapati

Abstract: The current investigation examined the effects of L-Carnitine on Kankrej bull semen, focusing on both semen characteristics and oxidative stress parameters. Results revealed that at the post-thawed (PT) stage, subjective motility, HOST reactive sperm, and sperm viability were significantly higher (p<0.05) in groups administered with 0.05 mg/ml and 0.1 mg/ml of L-Carnitine compared to the control group. Additionally, the percentage of total sperm abnormality at the post-thawed stage was significantly lower (p<0.05) in the 0.05 mg/ml and 0.1 mg/ml L-Carnitine groups compared to the control group. During post-dilution, post-equilibration, and post-thawed stages, no significant differences were observed in malondialdehyde (MDA) concentrations between the control group and those treated with 0.05 mg/ml and 0.1 mg/ml of L-Carnitine. Similarly, superoxide dismutase (SOD) concentrations did not significantly differ between the control group and the L-Carnitine treated groups during post-dilution and post-equilibration stages. Moreover, alanine aminotransferase (ALT) enzymatic values did not significantly differ between the control group and the groups treated with 0.05 mg/ml and 0.1 mg/ml of L-Carnitine across post-dilution, post-equilibration, and post-thawed stages. In conclusion, incorporating L-Carnitine at a concentration of 0.05 mg/ml in the semen extender emerged as the optimal choice for enhancing the qualitative parameters of post-thawed semen in Kankrej bulls.