Vol. 9, Special Issue 1 (2024)

Pseudomonas aeruginosa is a gram-negative bacteria prevalent throughout nature (i.e. plants, soil, and seawater). It can withstand temperatures as high as 42°C and exist without oxygen. It is an opportunistic animal pathogen and the sixth most often isolated organism. One of the main organism that causes nosocomial infection. It was associated with numerous clinical conditions such as pneumonia, cystic fibrosis (CF), and burns or wound infections. Its capacity to form biofilms is a well-known virulence factor. This study was designed for the qualitative and quantitative assessment of biofilm production in P. aeruginosa. 65 samples were collected from various animal species (such as dogs, cattle, camels, horses, and buffalo) that had clinical symptoms. A total of ten (from cattle, one from sheep, one from goats, and one from dogs) P. aeruginosa isolates were recovered. All isolates have been evaluated for their ability to form biofilms using qualitative as well as quantitative methods. We observed that all isolates of P. aeruginosa recovered from hospital-acquired infections had a greater tendency to form biofilm, which may aid in their virulence.