Vol. 8, Special Issue 4 (2023)

An endocrine hormone called prolactin (PRL) aids in regulating the broodiness of hens. Additionally, prolactin prevents poultry from producing eggs. The study's goal is to examine the prolactin expression profile in female Indian white leghorn layer strain (IWI) and Nicobari chicken birds over a period of 60 weeks using RT-qPCR. Three birds from each group are slaughtered, their anterior pituitary glands' total RNA is extracted, and cDNA is produced. The RT-qPCR tests showed that the IWI layer strain has minimal prolactin expression and Nicobari chicken had the highest prolactin content at 60 weeks. It is possible to draw the conclusion that natural chicken strains produce fewer eggs than strains that are chosen for high egg production due to the higher expression of prolactin.