Vol. 8, Special Issue 4 (2023)

The study focused on isolating and identifying Streptococcus, a mastitis-causing organism, from raw milk samples obtained from different dairy farms. A total of 100 milk samples were collected, and microbiological testing was conducted to analyse the raw milk. Results showed that 27 samples (27%) tested positive for Streptococcus through cultural methods, while 33 samples (33%) tested positive using PCR assay. Further analysis of the samples revealed that out of 40 milk samples from organized dairy farms, 10 (25%) tested positive for Streptococcus through cultural methods. Among the 40 samples from unorganized dairy farms, 15 (37.5%) were positive for Streptococcus. For the 20 samples from individual farmers, 2 (10%) tested positive through cultural methods. Similarly, using PCR, 12 (30%) samples from organized dairy farms, 18 (45%) samples from unorganized dairy farms, and 3 (15%) samples from individual farmers tested positive for Streptococcus. Conducting microbiological testing is crucial in identifying the causative agents of mastitis and implementing appropriate control measures to eliminate the infection.