Vol. 8, Special Issue 4 (2023)

Author(s): Deepak Soni and Sneh Lata Chauhan

Abstract: Aflatoxins are a major threat owing to their high heat tolerance and ability to cause serious health implications. The most prevalent form of aflatoxins found in feed and feed products is aflatoxin B1 (AFB1). AFM1 and AFM2 are the hydroxylated metabolic by products of AFB1 and AFB2, respectively. Dairy products contain aflatoxin M1 because it attaches to milk proteins, particularly casein. Aflatoxin contamination of milk and similar products is a problem for public health. AFM1 is cytotoxic and can also result in DNA damage, mutation, and chromosomal changes. AFB1 is one of the most potent known hepato-carcinogens. A number of monitoring techniques have been employed to gather data and find aflatoxins. These range from simple tests like the ELISA and HPLC to more recent proprietary methods like the CLIA (chemiluminescence enzyme immunoassay method) or the use of an immunochromatographic approach. The difficult requirements to develop a novel analytical method are the method's sensitivity and repeatability.

DOI: 10.22271/veterinary.2023.v8.i4Sa.621