Abstract: The aim of this study was to determine whether birth type-single versus twin has effects?on some of the physiological measurements performed in Awassi ewes. Ten lactating ewes?with live lambs were used; they were selected randomly and divided into two groups according to the birth order: five ewes with single lambs and, five ewes with twin lambs. Blood samples were taken three days post parturition for hematological (WBC, RBC, Hb, PLT), biochemical (cholesterol, triglycerides, HDL, LDL, VLDL) and hormonal (FSH, LH, progesterone,?estrogen) parameters determination. Between a single and a twin?group, no significant differences in hematological findings were observed. However, biochemistry assays showed significant differences in triglycerides, HDL,?and VLDL levels, indicating possible metabolic differences associated with the birth mode. Nevertheless, levels of all reproductive hormones did not differ?between groups. Generally, although hematological and hormonal parameters were some comprising predictors. Significant attempts to determine normal ranges have been proved?in animal models and in male athletes.