Vol. 10, Issue 9, Part D (2025)

Author(s): D Reena, KR Hema Niveda and S Rangasamy

Abstract: This study evaluated the impact of hyaluronan supplementation on the in vitro maturation (IVM) and developmental competence of sheep embryos. Oocytes collected from abattoir-sourced ovaries were cultured in IVM media with or without hyaluronan. Maturation was assessed via cumulus cell expansion, followed by fertilization using cauda epididymal sperm. Presumptive zygotes were then cultured in synthetic oviduct fluid (SOF), with or without hyaluronan. Results showed significantly higher maturation rates in hyaluronan-treated oocytes compared to controls (88.45% vs 78.30%, P< 0.01). Similarly, cleavage rates and embryo yields were significantly improved in the hyaluronan-supplemented group (89.64% vs 55.79%, P< 0.01). These effects are likely mediated by hyaluronan’s role in cumulus expansion, sperm capacitation, fertilization and early embryogenesis. In conclusion, hyaluronan supplementation enhances both oocyte maturation and embryonic development in vitro, offering a promising strategy for improving reproductive efficiency and genetic conservation in sheep.

DOI: 10.22271/veterinary.2025.v10.i9d.2558