Vol. 10, Issue 6, Part B (2025)

Author(s): Nagendra Singh, Akash Singh, Payal Tiwari, Renuka Mishra, Hanuman P Yadav, Anuj Kumar, Akhil Patel and Mukul Anand

Abstract: Assisted Reproductive Technology (ART), particularly in vitro Embryo Production (IVEP), plays a crucial role in advancing animal reproduction and biotechnology. Assisted Reproductive Technology (ART), particularly in vitro Embryo Production (IVEP), plays a crucial role in advancing animal reproduction and biotechnology, which aim to maximize both the quantity and quality of oocytes retrieved for in vitro maturation, fertilization, and culture. Oocytes can be sourced from slaughterhouse-derived ovaries or from live animals, both of which have significant benefits. Ovum Pick-Up (OPU) has developed as a minimally invasive, reproducible procedure for collecting oocytes from living donors, with considerable advantages for genetic improvement and commercial embryo production. Abattoir-derived ovaries, on the other hand, are a cheap and easily accessible source, with oocyte retrieval methods including slicing, aspiration, puncture, dissection and slashing. This review highlights the comparative analysis of these oocyte sources and harvesting techniques to improve IVEP outcomes in domestic animals.