Vol. 10, Issue 4, Part D (2025)

Author(s): Murugavel Sokkalingam, Lakshmi Prasanth Thangavelu and Paritosh Joshi

Abstract: Vitronectin is an adhesive extracellular matrix glycoprotein. This multifunctional protein is involved in diverse physiological functions by binding to ligands like Complement components, Plasminogen, Somatomedin B, Heparin etc. besides binding to cells and microbes. Two sites were identified as the cell attachment site of human vitronectin viz. the amino terminal RGD motifs and the Carboxy terminal heparin binding domain. The details of cell binding regions in the goat vitronectin are not known, which has two RGD motifs unlike most other species. In this study, we studied the role of C-terminal heparin binding region in binding to vero cells and it revealed that the putative heparin binding region comprising of the amino acids 341 to 380 of goat vitronectin were not involved in cell adhesion.