Molecular detection of drug resistance and virulence related genes in avian pathogenic Escherichia coli (APEC) isolated from poultry
Author(s): Dushyant Madhukarrao Muglikar, IH Kalyani, Dhruv Desai, JM Patel, DR Patel, KK Sharma, Pushpa Makwana and JB Solanki
Abstract: This study examined the occurrence of drug resistance and virulence-associated genes in 25 isolates of avian pathogenic
Escherichia coli (APEC). A total of five genes linked to antimicrobial resistance
strA,
strB,
tetA,
tetB, and
blaTEM were selected based on the phenotypic resistance observed to the corresponding antibiotics. A relatively high prevalence rate (92%) was found for the
blaTEM gene. Moderate occurrence rates were recorded for
strB (64%),
tetB (56%),
tetA (44%), and
strA (40%).
In addition, these E. coli isolates were screened for virulence genes. The iucD gene exhibited the highest prevalence (68%), followed by kpsMT II (60%), iss (48%), tsh (44%), vat (40%), papC (32%), irp2 (32%), and astA (28%). The cva gene was not detected in any of the 25 isolates.
How to cite this article:
Dushyant Madhukarrao Muglikar, IH Kalyani, Dhruv Desai, JM Patel, DR Patel, KK Sharma, Pushpa Makwana, JB Solanki. Molecular detection of drug resistance and virulence related genes in avian pathogenic Escherichia coli (APEC) isolated from poultry. Int J Vet Sci Anim Husbandry 2025;10(4):204-207.