Vol. 10, Issue 3, Part A (2025)

Author(s): RB Kate, DM Muglikar, UM Tumlam, MS Budhe, PP Mhase, SN Jadhav and AK Barate

Abstract: The study involved the collection of 235 milk samples from cows. The milk samples were taken from sub clinically infected cows that were tested positive for the California Mastitis test, as well as from clinically proven mastitis cases of cows. All of the affected cows were treated in the past with various antibiotics, and the majority of these cases involved recurrent mastitis. Out of the 235 milk samples collected for mastitis in this study, 50 (21.27%) were tested positive for Candida species based on colony features on Chromogenic media In this investigation, the three species of Candida were isolated. Among these three species of Candida, the most frequently isolated was Candida albicans (N=22, 9.36%), followed by Candida tropicalis (N=18, 7.65%) and Candida krusei (N=10, 4.25%). Phenotypically confirmed isolates were subjected to Antifungal susceptibility testing. The results of the antimicrobial sensitivity test for Candida albicans, as documented indicated that ketoconazole exhibited the highest efficacy, inhibiting 21 out of 22 isolates (95.45%), followed closely by fluconazole, The antimicrobial sensitivity test for Candida tropicalis, revealed fluconazole as the most effective drug, inhibiting all 18 isolates (100%). Ketoconazole, on the other hand, was shown to be almost as effective, inhibiting 17 of the18 isolates (94.44%). As per the findings of the antimicrobial sensitivity test conducted on Candida krusei, fluconazole emerged as the most potent drug, inhibiting 90% (9 out of 10) isolates.