Vol. 10, Issue 2, Part E (2025)

Author(s): Kanishk P, Haresh PT and Malarmathi M

Abstract: This study aimed to screen the prevalence of Bovine Citrullinemia (BC) and Factor XI Deficiency (FXID) in six cattle breeds of Tamil Nadu, including Holstein Friesian cross, Jersey cross, and four indigenous breeds (Pulikulam, Barugur, Alambadi, and Umbalacherry). Genomic DNA was extracted from 120 blood samples and subjected to nested PCR for BC and allele-specific PCR for FXID. For Bovine Citrullinemia, 317 bp amplicons were produced using outer primers, while 199 bp fragments indicated the normal allele. Whereas 170 bp was not detected which is a mutant allele. Similarly, 244 bp fragments were amplified for the wild-type FXID allele, with no evidence of the 320 bp mutant allele. The absence of mutant alleles suggests a BC and FXID where not present in the studied cattle population. The study highlights the need of routine genetic screening of breeding bulls to prevent hereditary transmission and recommends expanded surveillance to support sustainable breeding practices.