Vol. 10, Issue 2, Part D (2025)

Author(s): Hassan F Mohammed and Ibrahim M Kamal

Abstract: This study aimed to isolate and identificate Enterococcus spp. from human, dogs and cats, a total of 100 human samples group (G)1 including (100 nasal and 100 oral swabs) were collected from pet animal (dogs and cats) owners when visiting the Baghdad Veterinary Teaching Hospital for their animals, and stool samples were collected from another group as a group(G)2 from private laboratories. The samples were collected from both gender and of different ages. A total of 150 samples including oral, nasal, and fecal were collected from dogs (25) and cats (25) from both gender and different ages and breeds, the study was done from the 2ed Nov. 2021 to the 28th April. 2022. Antimicrobial resistance profile indicated that human enterococci isolates were 100% resistant to Ceftriaxone, Clindamycin, and Gentamycin, while 93.54% resistant to Erythromycin, Azithromycin and Doxycycline, MDR were 100% with high-risk of the isolates, Dogs isolates were resistant 100% against Cefitrixone, Clindamycin, and Gentamycin, 75% to Azithromycin and Doxycycline, MDR were 100% with high risk, cats isolates were resistant to Cefitrixone, Erythromycin, Azithromycin, Doxycycline, and Gentamycin. Most of the isolates 74% could form biofilm using congo-red method and crystal violate assay methods, 77.77% of them were weak, and 22.22% were moderate. E. gallinarum were recorded 27.7% of them were moderate and 72.3% were weakly formed, E. faecium were 75% weak, and 25% were moderate. Whereas, E. faecalis 85.71% were weak and 14.28% were moderate.