Vol. 10, Issue 2, Part A (2025)

Author(s): Mansoor Nabi, Najeebul Tarfain, Isfaqul Hussain, FaheemUdin, Junaid Mehraj and Mahesh Kuchana

Abstract: Bovine mastitis, a significant inflammatory condition affecting the mammary gland, imposes severe economic burdens on dairy farming worldwide by reducing milk yield and quality. This study investigates the sensitivity and specificity of six chemical diagnostic tests for bovine mastitis—pH test, Whiteside test, modified Whiteside test, bromothymol blue test, chloride test, and California mastitis test (CMT)—to determine their efficacy under resource-constrained conditions. In this study, we analysed 200 milk samples, with 100 confirmed as mastitis-positive and 100 confirmed as mastitis-negative, using somatic cell count (SCC) as the reference standard for comparison. These tests are essential due to their affordability, simplicity, and suitability for on-site diagnosis, making them particularly valuable in low- and middle-income countries. The sensitivity of these tests ranged from 55% to 73%, and specificity varied between 70% and 78%. The study sheds light on the limitations of current mastitis diagnostic tests, highlighting the need for further development of tests that offer improved accuracy and reliability across diverse contexts under limited resource settings. This will ultimately contribute to better mastitis management and animal welfare in these regions.