Molecular detection of some intestinal protozoa isolation from farm soil
Author(s): Safaa M Kareem and Akeel Mohammed Al-Musawi
Abstract: Intestinal protozoan parasites are globally distributed and are related with both humans and animals. They are transmitted to humans by the consumption of food and beverages infected with parasite cysts and oocysts. The current study registered the percentage Intestinal protozoan in Hilla city microscopic and molecular diagnosis from different area of Babylon between (April of 2024 to April of 2025). collected (200 ) farm soil samples, the prevalence of intestinal protozoa forms the soil samples by the molecular detection show Giardia intestinalis recorded 71/200 (35.5%), Cryptosporidium spp was 63/200 (31.5% ) and Entamoeba histolytica recorded 46/200 (23%). The phylogenetic analysis identified five sequences related with the G. intestinalis A and B (PX289656, PX289657, PX289658, PX289659, and PX289660). Furthermore, the phylogenetic analysis revealed five Cryptosporidium spp sequences (PX279584, PX279585, PX279586, PX279587, and PX279588). Lastly, five Entamoeba histolytica sequences were identified (PX247889, PX247890, PX247891, PX247892 and PX247893).
