Vol. 10, Issue 12, Part D (2025)

Author(s): Siraj Ansari, Ravi Dabas, Shruti Dehru, Vinita Rajpurohit, Neha Kumawat, Suraj Dhankar, Ravi Kumar, Raguvaran R, Bhand Akshata and Neetu Kumari

Abstract: A seven-year-old murrah buffalo weighing approximate 380 kg was presented to the Referral Veterinary Polyclinic of ICAR-Indian Veterinary Research Institute (IVRI), Izatnagar with the complaint of sudden drop in milk yield, anorexia, red and swollen udder, yellow watery milk for past three days and history of calving six days back. Physical examination of udder revealed heat, pain on palpation, edema, red and swollen udder. The changes in milk included alkaline pH and yellow watery color of milk. On laboratory examination of milk, score for california mastitis test was +++ and somatic cell count was found to be 4.00 ×10⁵ cells/ml of milk. Hematological investigation revealed neutrophilic leukocytosis. On culture and isolation, the causative agent was isolated and identified to be Escherichia coli. Antibiotic sensitivity test revealed that Escherichia coli was found more sensitive to enrofloxacin, cefoperazone sublactum, gentamicin and moxifloxacin. Molecular confirmation for E. coli was done by PCR for the presence of eaeA gene. The therapeutic management of buffalo was done by using parenteral enrofloxacin, flunixin meglumine, multivitamin, intramammary cefoperazone, topical herbal gel and oral vitamin E and inorganic selenium supplementation. This case study deals with the management of clinical mastitis in buffalo associated with enteropathogenic strain of Escherichia coli.

DOI: 10.22271/veterinary.2025.v10.i12d.2814