Vol. 10, Issue 10, Part G (2025)

Raju, a phenotypically female, free-ranging, Domestic Shorthair (DSH) cat, presented with alopecia, submandibular lymphadenopathy, and chronic non-resolving skin lesions. Diagnostic tests confirmed Trypanosoma spp. infection alongside concurrent dermatophytosis. Treatment with diminazene aceturate, doxycycline, supportive therapy, and topical antifungals led to full clinical recovery. A planned ovariohysterectomy (OVH) revealed the absence of female reproductive structures and the presence of bilateral intra-abdominal and perivulvar testicular tissue, which was histopathologically confirmed as azoospermic testes. These findings were consistent with male pseudohermaphroditism. This case highlights the rarity of feline trypanosomosis and its unique coexistence with a disorder of sexual development (DSD). It underscores the importance of comprehensive clinical, laboratory, and surgical evaluations in atypical presentations. These findings contribute to the limited literature on feline trypanosomosis and disorders of sex differentiation in cats.