Investigation on micrometric traits and their association in testis of dog (Canis familiaris)
Author(s): Gayatri M Gadariya, Vishnudeo Kumar, Anil Sharma and Mahesh R Gadariya
Abstract: The present investigation dealt with micrometric traits and their association in stray dog testis (Canis familiaris). The study was conducted on the testes of 18 adult, healthy dogs captured and operated for sterilization at the Veterinary Hospital of Junagadh Municipal Corporation, Ivanagar, and Junagadh. Micrometrical observations were recorded on the H & E-stained section of the testis of the dog, using Carl Zeiss Zen (Blue edition) microscopic image analysis software. The samples were examined under a light microscope using x100 and then x400 magnification. The micrometrical parameters (?m) recorded in the study were viz., i) thickness of tunica albuginea ii) number of seminiferous tubules (per mm2), iii) diameter of seminiferous tubules, iv) height of germinal epithelium, v) number of Sertoli cells/tubule, vi) nuclear diameter of Sertoli cells, vii) number of Leydig cells (per mm2) and viii) nuclear diameter of Leydig cells. Overall mean values for corresponding traits were 528.65±53.11 µm, 14.46±1.22 per mm2, 178.92±6.41µm, 61.88±2.88 µm,7.08±0.34/tubule,10.54±0.25 µm, 255.51±25.34 per mm2 and 5.84±0.12 µm, respectively. The number of seminiferous tubules (per mm2) was negatively and highly correlated (r value, -0.71 to -0.73) with their diameter (µm) and the height of epithelium (µm). The diameter of seminiferous tubules (µm) was positively and moderately correlated with its height of the epithelium (µm), No. of Sertoli cell/tubule and nuclear diameter of Sertoli cell (µm), with r value ranging from 0.44 to 0.66. Height of the seminiferous tubules’ epithelium was also positively associated (R-Value 0.55 to 0.63) with the number of Sertoli cell/tubule and the nuclear diameter of Sertoli cells (µm). The number of Sertoli cells/tubule and their nuclear diameter were positively and moderately associated (R-Value, 0.44), while the number of Leydig cells/tubule and their nuclear diameter were negatively and lowly associated (R-Value, -0.277).
