Vol. 9, Special Issue 6, Part E (2024)

Author(s): Aparna P, Alfin Mariya, Arya Mohan, Suresh N Nair, Sujith S, Zarina Aziz and Nisha AR

Abstract: Hesperidin is a flavone-glycoside present in many plants and have multiple pharmacological uses. In this context, in silico and in vivo assessment of acute toxicity of hesperidin was done in Daphnia magna to address the ecotoxic potential. The various concentrations of hesperidin from 1000 µg/mL, 500 µg/mL, 100 µg/mL, 50 µg/mL, 25µg/mL, 10µg/mL, 5 µg/mL, 1 µg/mL, 0.5µg/mL and selected 10 healthy D. magna were introduced into each well of six well plate. Observations were made after 24 hrs of exposure and number of mobile or dead daphnids were noted at each point. The in silico LC50 were predicted using T.E.S.T Tool. In silico toxicity of D. magna, the LC50 were found to be 296.13 µg/mL and in in vivo toxicity of D. magna the LC50 were found to be 93.87µg/mL. From the study, it could be concluded that hesperidin induces cytotoxicity in D. magna in both in silico and in vivo tests.