Vol. 9, Special Issue 6, Part E (2024)

Author(s): D Reena, S Gangasudan, Priyanka Thammina and S Manokaran

Abstract: Presently, shorter co-incubation time of gametes during in vitro fertilization is widely used method and is said to have better outcomes. The present study aims to assess the effect of different time duration of co-incubation on the in vitro embryo development of buffalo gametes. For the study, a total of 361 oocytes recovered from the 83 buffalo ovaries by slicing method and matured in vitro for 24h. After maturation, oocytes were co-incubated with motile sperms for 6 and 18h. The presumptive zygotes were cultured in SOF culture system. The cleavage rate of 68.42 (99/145) and 70.67 (102/145) was obtained in 6 and 18h group, respectively. The percentage of compact morula was significantly higher (p<0.01) in 6h co-incubation group (23.27%) than the 18h group (13.71%). Results of the present study indicated that shorter co-incubation time might be used to increase the In vitro embryo production.