Vol. 9, Special Issue 6, Part C (2024)

Author(s): N Sweetline Anne, TV Meenambigai and S Manoharan

Abstract: A total of 40 rectal swabs was collected from healthy piglets of about 6 weeks old belonging to three different piggery farms from Kanyakumari district. All the samples were processed and subjected to the screening and detection of the PCV2 genome by partial amplification of the ORF2 gene which codes for capsid protein and serves as an epidemiological marker. In that, about 20 samples were positive for PCV-2. Randomly, 04 partially amplified ORF2 genes were sequenced and the aligned sequences were subjected to BLAST analysis and phylogeny in MEGA 11. BLAST analysis showed that they are having homology with PCV 2b genotypes sequences. The PCV2b genotype has 99.9% sequence homology with Chennai, Kerala and Punjab isolates of India followed by 99.3% homology with China and Vietnam isolates. This study emphasize the importance on increase in subclinical porcine circovirus -2 infection to make effective measures to control over the disease.

DOI: 10.22271/veterinary.2024.v9.i6Sc.1895