Vol. 9, Special Issue 6, Part A (2024)

Author(s): Dr. M Anandhi

Abstract: The current study was aimed to ascertain the effect of in-ovo injection of nano particles of zinc, copper and chromium on intestine and lymphoid organs development of broiler chicken. Four hundred fertile broiler eggs from Cobb 430 were randomly divided into five treatment (each 80 eggs) groups. First treatment was without injection (control), second was injected with 0.5 ml normal saline, third, fourth and fifth treatment eggs were injected with 40, 12 and 0.5 µg/egg of nano zinc, nano copper and nano chromium, respectively. After hatching, 240 day old straight run broiler chicks were allocated to five treatment groups each consisting of four replicates with twelve chicks per replicate and all birds were fed with broiler starter diet (1-21 days) and finisher diet (22-42 days) as per NRC (1994)^[11]. In-ovo feeding of nano forms of zinc, copper and chromium at 18^th day of incubation through amniotic route significantly (p≤0.05) increased the histomorphometry of small intestine at early age and increased the lymphoid organs weight of broilers.