Vol. 9, Issue 6, Part K (2024)

Author(s): Murugavel Sokkalingam, Lakshmi Prasanth Thangavelu and Paritosh Joshi

Abstract: Site directed mutagenesis is a powerful technique for studying gene and protein functions by altering the nucleotide bases at the genome level. Many methods are employed for mutagenesis of the desired genes. Polymerase chain reaction is one of the commonly used methods in recent days for substitution, deletion and addition of bases to a nucleotide sequence creating the desired mutations in the target gene sequence. Human Vitronectin, is a glycoprotein present in the extracellular matrix, having a domain structure and organisation, whereas the details of goat vitronectin are not clear. In the current study, deletion mutants of the putative heparin binding region of goat vitronectin were generated, which could be used for further functional studies of the protein; further a simple, PCR amplicon size based mutant clone detection for heparin binding region of goat vitronectin is reported.