Vol. 9, Issue 6, Part J (2024)

Author(s): Leeba Chacko, Vigneshwaran B, K Promod, Renjith Sebastain, Magnus Paul K, Muhammad Aslam MK, R Rajeev, Bineesh C Vijay and Anupam Arun

Abstract: Cryopreservation of Malabari buck semen provides valuable genetic preservation, but its effectiveness is hindered by cryodamage, with low-freezability (LF) bucks being particularly susceptible. This study evaluated fresh and post-thaw sperm quality in high-freezability (HF) and LF bucks. HF bucks demonstrated significantly higher post-thaw motility (47.50±1.53% vs. 15.75±1.16%), viability (78.45±0.41% vs. 73.35±0.97%), acrosomal integrity (62.65±0.91% vs. 44.60±1.67%), and hypo-osmotic swelling (HOS) response (62.65±0.92% vs. 38.65±1.91%) compared to LF bucks (p < 0.05). DNA integrity (57.10±1.08% vs. 35.60±1.82%) was also superior in HF bucks. Malondialdehyde (MDA) levels, indicative of oxidative stress, were lower in HF bucks (7.88±0.33% vs. 11.48±0.29%). These findings highlight the resilience of HF bucks and the importance of selective breeding and optimising cryopreservation protocols to enhance artificial insemination outcomes in Malabari bucks.

DOI: 10.22271/veterinary.2024.v9.i6j.1953