Vol. 9, Issue 6, Part J (2024)

Author(s): Saharuz Zaman Laskar, Dibyajyoti Talukdar, Ajoy Ghosh, K Lalrintluanga, Fazal Ali Ahmed, Girin Kalita, TC Tolenkhomba and Bedanga Konwar

Abstract: Artificial insemination (AI) has become the preferred reproduction method in modern pig farming, offering several advantages, including faster genetic improvement, wider use of superior male germplasm, and long-distance semen transport. However oxidative stress during semen storage can negatively impact sperm function and fertility by damaging proteins, lipids, and nucleic acids. The present study evaluates the preservation and fertility outcomes of Large White Yorkshire boar semen stored at 18°C using Beltsville Thawing Solution (BTS). Parameters such as sperm motility, viability, abnormalities, intact plasma membrane, intact acrosome and lipid peroxidation were evaluated Immediately after semen collection across 40 ejaculates. Results revealed significant variation in semen volume, motility, and live sperm percentages among boars. Post 24-hour of preservation, motility and viability declined but remained within usable ranges. Double-dose insemination did not significantly enhance litter size compared to single-dose. No significant differences in litter size were observed between single and double-dose inseminations but there was a slight increase in litter size in double insemination group.