Vol. 9, Special Issue 5, Part F (2024)

Hydropericardium hepatitis syndrome is a newly emerging disease of poultry with sudden occurrence of high mortality. The CPE produced were more marked after 5-6^th passages. Cytopathic effect due to the growth of CEL adopted FAV was observed from 24-76 hrs of post infection. Clumping, rounding and fusion of cells were observed during early stages followed by shrinkage of monolayer spaces and formation of inclusion bodies from the cells as reported earlier as cytopathic effect advances, multinucleated giant cells and vaculation in the cytoplasm often observed compared with normal uninfected cells. The physical characterization of FAV 4 was done at different temperatures and different ranges of pH and the virus was stable in 50-70% chloroform, 50-70% ether, 0.25% trysin, heat at 50 °C but labile to 100% ethanol, acidic condition at 3p^H and heat at 52 °C, 1M of Mgcl₂ at 50 °C for 1 hour, 1:2000 formalin at 37 °C with the TCID₅₀ 50 oC for 15 hours. The nonenveloped FAV 4 was not sensitive to lipolytic agents such as chloroform and ether.