CONTACT: +91-9711224068
NAAS Journal
  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal

Vol. 9, Special Issue 5, Part B (2024)

Detection of dermatophytes through multiplex PCR and loop-mediated isothermal amplification (LAMP)

Author(s): AP Surendar, M Vijayabharathi, NR Senthil, S Sureshkannan and S Parthiban
Abstract: Dermatophytes are zoonotic fungal agents that are infectious and widespread among animals globally. The new loop-mediated isothermal amplification (LAMP) method and Multiplex PCR are tools for identifying these agents. Both offer high specificity and sensitivity, and are simple and quick to execute. The aim is to develop a LAMP and a rapid multiplex PCR method to detect Microsporum spp and Trichophyton spp, the most commonly isolated fungal genera from cats and dogs. Both methods focus on the CHS-1 gene. They were evaluated for specificity and sensitivity using 34 and 43 cultures obtained from dogs and cats, in that 21 Microsporum and 8 Trichophyton strains from dogs, and 29 Microsporum and 12 Trichophyton strains from cats. The specificity was 85.29% and 95.34% for dogs and cats, the sensitivity for 89% M. canis and 85% T. mentagrophytes respectively. The LAMP and multiplex PCR required 60 and 140 minutes, respectively, for both targets. The limit of detection (LOD) for both was 10 spores/mL for LAMP and 1 spore/mL for multiplex PCR.
Pages: 76-78  |  124 Views  8 Downloads


International Journal of Veterinary Sciences and Animal Husbandry
How to cite this article:
AP Surendar, M Vijayabharathi, NR Senthil, S Sureshkannan, S Parthiban. Detection of dermatophytes through multiplex PCR and loop-mediated isothermal amplification (LAMP). Int J Vet Sci Anim Husbandry 2024;9(5S):76-78.
Call for book chapter
International Journal of Veterinary Sciences and Animal Husbandry