Vol. 9, Special Issue 5, Part A (2024)

Author(s): Seema B Gadhavi, Bharat B Bhanderi, Manasi M Soni and Khushbu S Rana

Abstract: Staphylococcus species, gram-positive cocci, are classified into coagulase-positive staphylococci (CoPS) and coagulase-negative staphylococci (CNS), causing infections in both animals and humans. This study included 100 staphylococcal isolates from mastitis (n=32) and wound infections (n=68) in goats, dogs, cattle, horses, and buffalo. Species identification was performed using sugar fermentation and PCR tests. Sugar utilization patterns identified S. aureus (63%), S. intermedius (22%), S. pseudintermedius (10%), and S. epidermidis (5%). PCR tests showed 94% genus-level positivity, identifying S. aureus (58%), S. intermedius (19%), S. epidermidis (9%), S. delphini group A (2%), and S. schleiferi subsp. coagulans (1%). CoPS comprised 80% of isolates, CNS 9%, with 5% unidentified. Species-level agreement between both tests was 77%, with 23% variation. The study highlights the significance of species-level identification and the role of S. delphini group A and S. schleiferi subsp. coagulans in animal infections.

DOI: 10.22271/veterinary.2024.v9.i5Sa.1651