Vol. 9, Special Issue 4, Part C (2024)

Author(s): P Ponnusamy, T Lurthu Reetha, BSM Ronald, R Manickam and B Puvarajan

Abstract: Canine papillomavirus causes papillomas and plaques on the skin and mucous membranes of different sites. The present study aimed to identify the genotype of the canine papillomavirus associated with oral papillomatous growth. A six month old male Rottweiler was carried to Veterinary College and Research Institute, Orathanadu with a history of wart like growths protruding from the oral cavity. The growths suspected of papilloma from the oral mucosa were removed surgically for detection of canine oral papillomavirus (COPV) by PCR. The PCR was carried out by amplifying the portion of early (E6, E7) and late genes (L1) of COPV by using respective gene specific primers. Further, nucleotide sequencing and phylogenetic analysis of early (E6, E7) and late genes (L1) revealed that the sequences were closely related with previously reported CPV 1 belonging to the Lambdapapillomavirus genus. The present study concluded that the CPV 1 was the cause of papillomatous growths which is mostly associated with non-neoplastic papillomas in the oral cavity.