Vol. 9, Issue 4, Part F (2024)

Author(s): Dr. Dinesh V Chaudhari, Dr. Dignesh K Patel, Dr. Bhakti P Chavda, Dr. Mitesh P Patel, Dr. Rutvij B Patel and Dr. Prachi Sharma

Abstract: This study evaluated the cryoprotective effects of epigallocatechin-3-gallate (EGCG) in a tris-citrate-fructose-yolk-glycerol (TFYG) extender on cryopreserved Murrah buffalo bull semen. Semen samples were extended with 0, 0.2, 0.4, and 0.6 mg/ml EGCG. Post-thaw analysis showed that 0.4 mg/ml EGCG significantly improved sperm motility (54.58±1.57%), viability (60.92±1.32%), and plasma membrane integrity (45.83±1.87%) compared to control. EGCG at 0.4 mg/ml was the most effective concentration, enhancing antioxidant defense and reducing oxidative stress-induced damage. These findings suggest that EGCG supplementation in semen extenders can enhance the quality of cryopreserved buffalo semen, thereby supporting artificial insemination and genetic conservation efforts.