Vol. 9, Issue 4, Part D (2024)

Author(s): KV Shinde, PM Kapale, SP Waghmare, DM Badukale, SW Hajare, AG Ginode, GM Gadegaonkar and AN Solanke

Abstract: The present study was carried out to evaluate the effect of cow urine distillate fortified Curcuma amada extract therapy on subclinical mastitis (SCM) in cows. The study was conducted on cows at dairy farms. Thirty cows in the mid-lactation stage were randomly selected and divided into five equal-treatment groups viz T₀ was control, T₁ group was treated with Cow urine distillate @0.15 ml/kg body weight diluted in water by oral administration (post-milking), T₂ was given Curcuma amada aquous extract, T₃ was treated with Curcuma amadaaquous extract @20 mg/kg b.w.t diluted in water by oral administration (post-milking) for 28 days. Group T₄ treated with morbofloxacin @8mg/kg BW IM single dose (post-milking). The California mastitis test (CMT) was conducted on total 52 cows out of which 24 animals were found positive for SCM. The SCM prevalence was 46 percent. The study revealed significant alterations in the physical properties of milk samples from subclinical mastitis cows, including increased pH, total protein, albumin, and lactate dehydrogenase enzyme levels, alongside decreased milk fat, density, and viscosity. Hematological parameters also showed significant changes, with increased total white blood cell count, monocytes, and granulocytes, and decreased hemoglobin, packed cell volume, total red blood cell count, and lymphocytes. The anti-mastitic activity of CUD fortified Curcuma amada extract was found better than CUD or C. amada alone treated groups. However, the group which received standard drug morbifloxacin showed better anti-mastitic activity in SCM affected cows. The study demonstrates the efficacy of cow urine distillate and Curcuma amada extract in treating subclinical mastitis, offering a promising solution to this prevalent issue in the dairy industry.