Vol. 9, Issue 2, Part N (2024)

Author(s): Monica Gracia Hetharia, I Nyoman Sulabda and I Ketut Puja

Abstract: The success of artificial insemination in dogs is influenced by many factors, including good semen preparation and handling and the number of sperm cells per dose. Good semen quality is influenced by many factors, one of which is the frequency of ejaculation. Ejaculation frequency that is too frequent will cause a decrease in libido and semen quality. This study aimed to compare semen quality parameters (motility, viability, concentration) during ejaculation rest periods of between 3 days and 5 days. A total of four Balinese Kintamani dogs were divided into 2 groups, namely Group A whose semen was taken on day 0 and between the 3rd day, and Group B whose semen was taken on day 0 and between the 5^th day. The results showed that the average spermatozoa concentration on day 3 and day 5 was 3102 ± 1.74 and 5712 ± 1.72 million/ml, respectively. The average motility taken on day 3 and day 5 respectively was 90.13 ± 1.50 and 92.88 ± 1.62 and the average viability on day 3 and day 5 respectively was 87.75±1.61 and 93.06±1.61. The results showed that there was an increase in semen quality during the 5th day of ejaculation rest. In conclusion, the ejaculation rest period on day 5 showed better semen quality compared to the ejaculation rest period on day 3.