Vol. 9, Issue 2, Part L (2024)

Author(s): G Shalini, D Antoine, S Kantharaj and K Murugavel

Abstract: Biochemical evaluation of seminal plasma is important aspect of semen evaluation. Spermatozoa's capacity to fertilize is regulated by seminal plasma, a complex mixture of secretions derived from the testis, epididymis, and accessory sex glands. Seminal plasma proteins support capacitation, spermatozoa adhering to the oocyte's zona pellucida and spermatozoa survival and viability in the female reproductive system. The canine seminal plasma proteins also function as signaling molecules for the female immune System, influencing the female genital tract's ability to reject or tolerate spermatozoa. A total of 22 dogs aged between 2-6 years were selected for semen collection and evaluate the biochemical parameters of canine ejaculate. Semen was collected by digital manipulation at weekly interval for six consecutive weeks. A total of 115 ejaculates from eight different breeds of dogs. The colorimetric method was used to estimate the total protein, albumin, globulin, ALP, and ACP. The biochemical attributes of semen for fertile and infertile dogs were characterized and compared. The ALP and ACP level in the seminal plasma were significantly low in infertile dogs compared to fertile dogs. The observed notable variations in ALP and ACP levels among the groups may be attributed to dysfunction in the prostate gland and epididymis, as these biochemical markers indicate the functional integrity of the gland and epididymis, respectively. The percentage of fertile and infertile dogs in the present study was 59% and 41% respectively.