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Vol. 9, Issue 2, Part K (2024)

Molecular detection of Escherichia coli and Shiga toxin-producing genes stx1 and stx2 from diarrheal samples in dogs

Author(s): Shimaakhtar Saiyad and Bharat Bhanderi
Abstract: Diarrhoea is a widespread public health concern that can be caused by several pathogens, such as Salmonella, Shigella, Escherichia coli, etc. Escherichia coli is found in both animals and humans. Multidrug-resistant E. coli bacteria with varying antibiotic resistance profiles, especially for Extended-spectrum beta-lactamases (ESBL), can be found in pet dogs. Infant diarrhoea, hemorrhagic colitis, thrombotic thrombo cytic purpura, and hemolytic uremic syndrome are all linked to Shiga-like toxin-producing E. coli (SLT-EC). This study aimed to molecularly identify E. coli from diarrheal samples of dogs from Anand, GJ and detect Shiga toxin-producing genes stx1 and stx2 using PCR technique. 58 out of 68 rectal samples were found to have the presence of E. coli using PCR using species-specific primers. In this study, the overall prevalence of E. coli was 85.29%.
Pages: 767-769  |  54 Views  3 Downloads


International Journal of Veterinary Sciences and Animal Husbandry
How to cite this article:
Shimaakhtar Saiyad, Bharat Bhanderi. Molecular detection of Escherichia coli and Shiga toxin-producing genes stx1 and stx2 from diarrheal samples in dogs. Int J Vet Sci Anim Husbandry 2024;9(2):767-769. DOI: https://doi.org/10.22271/veterinary.2024.v9.i2k.1288
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International Journal of Veterinary Sciences and Animal Husbandry