Vol. 9, Issue 2, Part I (2024)

Author(s): Rutvij Patel, Jainik Prajapati, Vishal Suthar, MM Mansuri, Dhwani Jhala, Sanman Samova, BN Suthar, Madhavi Joshi, Chaitanya Joshi and HC Nakhashi

Abstract: This study was conducted on buffalo oocytes collected from slaughtered ovarian specimen to evaluate the effect of season and presence of corpus luteum (CL) on In vitro maturation rate along with examining the survivability of oocytes after processing for vitrification and thawing (devitrification). COCs were aspirated from surface follicles after noting the presence of CL on ovaries. Maturation rate was compared between winter (November to February) and summer (March to June) seasons. Out of total 944 COCs, 830 (87.79±0.9%) COCs have shown the In vitro maturation. Maturation rate in winter and summer season was 89.76±1.08 (437/486) and 85.35±0.56 (391/458) per cent, respectively. Maturation rate in COCs collected from the ovaries with CL and without CL was 82.98±1.22 (292/348) and 90.34±1.25 (536/596), respectively. Oocytes were vitrified using vitrogen vitrification kit according to different time exposure and stored in liquid nitrogen (LN₂). Thereafter, oocytes were devitrified, stained with Hoechst 33342 staining and evaluated for viability under inverted microscope in fluorescent light. A total of 112 devitrified and 63 fresh oocytes were examined and 50.89% (57/112) and 96.83% (61/63) live oocytes among them, respectively. This study concludes that no effects of factors like season or morphological structure like CL was observed on COCs recovery or maturation and revival rate of vitrified COCs was not up to the mark and needs to be reinvestigate with modification of vitrification media.