Vol. 9, Issue 2, Part G (2024)

Author(s): MU Prajapati, HC Nakhashi, AS Nair, DT Jogdand, HG Raval and BS Rathod

Abstract: This study applied nine artificially collected ejaculates from three mature Kankrej bulls to study the cryopreservation of semen from mature Kankrej bulls in a tris egg yolk fructose dilutor at varying concentrations (G I: control, G II: 1.5 mM, and G III: 2 mM) of vitamin-E and its impact on spermatozoa penetration within cervical mucus and sperm morphometry. Fresh semen was found to have a sperm mucus penetration distance (SMPD) of 16.78±0.84 mm. Nevertheless, during post-thaw stages of cryopreserved Kankrej bull semen, the overall mean penetration distance of cryopreserved bull spermatozoa was determined to be 19.89±0.63 mm in G I, 26.44±0.82 mm in G II, and 23.00±0.69 mm in G III vitamin-E group, respectively. Compared to other concentrations, the SMPD was considerably (P < 0.01) greater in 1.5 mM vit-E. Fresh semen was discovered to include spermatozoa with average head length (HL), head width (HW), and midpiece length (ML) measurements of 8.62 ± 0.04, 4.52 ± 0.03, and 13.98 ± 0.05 μm, respectively. Using different quantities of vitamin-E at the post-thawed stage, the overall mean HL, HW, and ML were 8.56±0.03, 4.44±0.05, and 13.75±0.07 in G I; 8.51±0.03, 4.43±0.04, and 13.72±0.05 μm in G II; and 8.52±0.05, 4.45±0.08, and 13.74±0.06 μm in G III group, respectively.

DOI: 10.22271/veterinary.2024.v9.i2g.1243