Vol. 9, Issue 2, Part G (2024)

Author(s): Parth H Agrawal, MN Brahmbhatt, JB Nayak, BC Parmar, JH Chaudhary and AP Pachpinde

Abstract: In the present study, 165 Chevon samples were gathered from multiple meat shops located in the vicinity of Anand city. Among these 165 specimens, 48 tested positive for Bacillus cereus using selective media Polymixin egg-yolk mannitol bromothymol blue agar (PEMBA), that displayed typical bluish growth surrounded by lecithinase activity. All isolates underwent catalase test and VP test and were found positive, and a majority of them also tested positive for nitrate and exhibited motility. Tests for sugar fermentation depicted variability. All isolates underwent testing against a panel of six antibiotics. Antibiotic susceptibility testing revealed Meropenem, Imipenem, and Clindamycin as the most effective. PCR confirmed B. cereus identity using the groEL gene. Virulence gene analysis showed the presence of gyrB, bceT, hblA, and cytK, indicating potential toxin production and cytotoxicity.