Vol. 9, Issue 2, Part D (2024)

Author(s): Gavali Sandeep, Karande Vikas, Ghadigaonkar Sushma and Joshi Purva

Abstract: The study was planted to assess the in vitro antibacterial properties of ethanolic and aqueous extracts of Turmeric (Curcuma longa), Pudina (Mentha arvensis), Garlic (Allium sativum), Tulsi (Ocimum sanctum), and Curry leaves (Murraya koenigii), against Multi Drug Resistant strains of S. aureus sourced from bubaline mastitis. Concentrations of 2.5, 5.0, and 10mg/100μL were tested using agar well diffusion, while Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were determined via broth dilution and MSA plates respectively. Ciprofloxacin served as the standard antibiotic, showing an 18.2 ± 0.66mm zone of inhibition. Ethanolic extracts from all plants, except Garlic, exhibited superior activity compared to Ciprofloxacin and aqueous extracts. Ethanolic extract of Turmeric showed the highest activity with a 26.4±2.42mm zone of inhibition. Pudina, Curry leaves, and Tulsi had moderate activity with 22±2.09, 22.2±1.82, and 22.8±2.26mm zones of inhibition at a 10mg/100ml concentration. Ethanolic garlic extract had lower activity, with a 12±0.83mm zone of inhibition at the same concentration. Aqueous extracts were less effective than ethanolic ones at all concentrations with statistically significant differences (p<0.01).

DOI: 10.22271/veterinary.2024.v9.i2d.1203