Vol. 9, Issue 2, Part C (2024)

Author(s): PD Bhole, Rupali Charjan, SB Banubakode, NC Nandeshwar, UP Mainde, Anand Singh and Ayesha Boddupalli

Abstract: This study is aimed to utilize Polymerase Chain Reaction (PCR) techniques for species and sex identification in domestic animals. By targeting specific genetic markers, namely the Cytochrome b gene for species identification and the SRY gene for sex determination, along with the GAPDH gene as a positive control, the study successfully differentiated between various domestic ruminant species and determined the sex of individual animals. Results revealed distinct band patterns corresponding to different species, with band sizes of 106 bp, 163 bp, 232 bp, and 308 bp for buffalo, cattle, goat, and sheep, respectively. Additionally, the duplex PCR of SRY and GAPDH genes exhibited two bands at 122 bp and 218 bp for male individuals, while female individuals displayed a single band at 218 bp. This study concludes that the Cytochrome b gene primer effectively identifies ruminant species, while the duplex PCR of SRY and GAPDH genes accurately determines the sex of individual animals. These findings underscore the utility of PCR techniques in facilitating precise species and sex identification in domestic animals.