Vol. 9, Issue 2, Part C (2024)

Author(s): PD Bhole, Rupali Charjan, SB Banubakode, NC Nandeshwar, UP Mainde, Anand Singh and Ayesha Boddupalli

Abstract: Identification of species of animals from a very small size of recovered biological sample from the site of crime has received great importance in vetero-legal cases to curb the practices of cruelty against animals and to lock up criminals. DNA is used to identify Buffalo, Cattle, Sheep, and Goat species. Six samples of each species were collected and DNA was extracted. The obtained DNA was then subjected to PCR, by using the species-specific Cytochrome b gene as a marker. The processed PCR outcome was electrophoresed in agarose gel and illuminated with a UV transilluminator. The illuminator revealed fragments at 106 bp, 163 bp, 232 bp, and 308 bp for Buffalo, Cow, Sheep, and Goat, respectively.