Abstract: Objective: The research was structured to investigate the resistance patterns of
S. aureus isolates from Bareilly, India, particularly concerning tetracycline and quinolone resistance.
Methods: Standard microbiological techniques were utilized to isolate and characterize the S. aureus strains. Isolates were subjected to ABST. PCR was employed to detect and characterise the genes associated with tetracycline and quinolone resistance.
Results: Around 164 isolates of staphylococci isolated from mastitic milk, wound, and street food samples were used for the study. Out of 164 isolates, 77 (46.9%) were confirmed as S. aureus. Among 77 S. aureus isolates, 14% and 43% isolates were resistant to doxycycline and ciprofloxacin, respectively. Almost 50.6% of S. aureus isolates were multidrug resistant. Genotypic detection of AMR in S. aureus isolates revealed that 47%, 11.7% and 5.8% of doxycycline resistant isolates were positive for tetK, tetM and tetL genes. The tetM gene was significantly more common in S. aureus causing wound infections while no significant difference was apparent for occurrence of tetK and tetL carrying S. aureus of different origin.