Vol. 9, Issue 1, Part T (2024)

Author(s): Aathira KK, Suja Rani S, Vadlakunta Chetna, Anoopa Satheesan, Nisha AR, Bibu John Kariyil, Anoopraj R and Uma R

Abstract: Beta-caryophyllene is a natural odorous bicyclic sesquiterpene found in various essential oils known for its anti-inflammatory, antioxidant, analgesic and wound healing properties. In this study, in silico docking and in vitro analyses have been used to appraise the wound healing potential of beta-caryophyllene (BCP). Molecular docking analysis was performed with Auto Dock V 4.0 using BCP against five receptors such as TGF- β1 (PDB ID- 1PY5), TGF- β2 (PDB ID- 1M9Z), VEGF (PDB ID-3QTK), GSK-3β (PDB ID-1Q5K) and MMP-9 (PDB ID-5UE4) and the binding energy was determined. Cytotoxicity of BCP was evaluated using MTT assay and cell migration ability of BCP was assessed using scratch assay in L929 mouse fibroblast cell lines. In silico screening identified strong binding affinities between BCP and crucial wound healing targets with binding energy ranging from -6.84 Kcal/mol to -3.77 Kcal/mol with highest negative binding energy with GSK-3β endorsing its anti-inflammatory property. Subsequent in vitro investigations demonstrated the non-cytotoxic nature of BCP up to 320 μM concentration, validating its safety profile. Furthermore, in scratch assay, BCP exhibited a distinguished induction of cell migration in L929 mouse skin fibroblasts with 19.78%, 34.32%, 91.34% wound closure at 12hrs, 24hrs and 48hrs of incubation respectively, emphasizing its positive impact on wound healing processes. This study provides valuable insights into the molecular interactions of BCP and highlights its pledging role as a potential wound healing agent, offering a foundation for further research and therapeutic development.

DOI: 10.22271/veterinary.2024.v9.i1t.1155