Vol. 9, Issue 1, Part Q (2024)

Author(s): Arun TS and VK Saxena

Abstract: Metagenomics is the culture independent analysis of microbial diversity in an ecosystem and it can be used to find out the gut microbial diversity of chicken. Native chicken is characterised by good genetic resistance and they are adapted to extensive system of management. NGS analysis of intestinal micro biome using primers targeting V₃, V₄ and V₄-V₆ region of 16S rRNA helped in revealing the microbial diversity of native chicken CARI Nirbheek. Comparison of gut microbial diversity under two management systems viz., intensive and extensive system was done using MG-RAST. Lactobacillus was the dominant genus under both systems of management. Lactobacillus sakei was the dominant bacterial strain under intensive system and Lactobacillus helveticus was dominant under extensive management. The present investigation helped in exploring the intestinal microbial diversity and identifying the bacterial strains which helps in increasing the genetic resistance and growth of native chicken under backyard rearing.