Vol. 9, Issue 1, Part P (2024)

Author(s): Y Nagendra Reddy and G Shalini

Abstract: Among the assisted reproductive technologies Ovum Pick-Up (OPU) and In Vitro Embryo Production is potential alternative traditional in vivo embryo production mainly for the conservation of indigenous cattle breeds, augmentation of productivity and exploitation reproductive potential superior cows. OPU we can collect more oocytes from genetically superior animals and in turn production of a greater number of embryos per donor animal with subsequent fresh transfer of embryos or cryopreservation of embryos for frozen transfer and finally production of superior elite animals. This can be accomplished by selection of donor animals for OPU and donor preparation by stimulation with or without gonadotropins, efficient culture media and fresh transfer embryos. OPU can performed pre pubertal animals, pubertal calves, non- pregnant animals and even pregnant animals up to three months of gestational age. Present review emphasizes mainly on the history of OPU, factors affecting the OPU, donor preparation, OPU procedure and OPU complications.