Vol. 9, Issue 1, Part N (2024)

Author(s): Dr. Manoj Kumar, Dr. Ashwni Kumar Pandey, Shiv K Tyagi, Sonal Sharma, Dr. Sudarshan Mahala and Chirag Chaudhari

Abstract: Foot-and-Mouth Disease (FMD) poses a significant economic threat globally, with estimates of annual losses ranging from 12,000 to 14,000 crore FMD in India alone. The study evaluates the expression profiles of key immune response genes CCL8, CXCL10, and IFIT2 in Tharparkar cattle post-FMD vaccination. PBMCs samples were collected from the blood at three time points: pre-vaccination (day 0), and post-vaccination on days 7 and 21 for the study and gene expression was analyzed using quantitative real-time polymerase chain reaction (ORT-PCR). Results indicate an upregulation of CCL8 on day 7 and day 21 post-vaccination, aligning with previous findings after FMD vaccine and challenge studies. Similarly, CXCL10 showed an initial upregulation on day 7, however, a downregulation was noted on day 21. IFIT2 exhibited a complex expression pattern, with a decrease on day 7 and on day 21, differing from previous studies. The results provide preliminary insights into the Tharparkar breed's unique response to vaccination, emphasizing the need for further validation with a larger sample size.