Vol. 9, Issue 1, Part N (2024)

Author(s): Anju Chaudhary, Surendra, Sudeep Solanki and Durga Gurjar

Abstract: The dairy sector and farms have been seriously threatened by calf diarrhea. One of the main causes of this multi-etiology sickness is Salmonella spp. The goal of this work is to characterize Salmonella spp. that have been isolated from calf diarrhea biochemically. A total of 100 fecal samples were collected from calves of cattle and buffalo of below 3 months of age. Sterile fecal swabs (Hi-media, India) were used for sample collection. All the samples were transported immediately to the laboratory under cold chain and were processed for biochemical characterization of Salmonella spp. For isolation and identification of Salmonella spp. a loopful of fecal sample was enriched in Selenite broth and incubated at 42 °C for 12-14 hours. After that a loopful of selenite broth was inoculated and streaked separately onto selective agar plates as xylose Lysine Deoxycholate (XLD) agar, Brilliant Green agar (BGA), MacConkeyꞌs agar and incubated at 37 ℃ for 24hours. Partially identified cultures were suspended in normal saline and smeared over a slide. The smears were allowed to air dry followed by heat fixing and Gram’s staining. All Gram negative culture was subjected to biochemical characterization by using various biochemical tests such as catalase, oxidation, oxidation-fermentation, Indole, methyl –red, Voges-Poskauer, citrate test, TSI and urease test. The overall prevalence of Salmonella spp. in the present study was recorded 3%.